Clone

Target

IDP00181  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

1119  

Reference Sequence Used

MLRSSYVRPI ISWSFGLLLS FFLLMPLHAA TQPNEEQVRQ ELKLAESNKT TPNQAEIVQA LQGALSWLAD AKESDIRAQQ YQKAIDDFPK LTRELRQQLA QEGDKPLPVP SNLSTSDLEQ QVLQVSSQLL ELNRLSQQEQ DRAREISESL GQLPQQQSEA RRMLAEIGPR IQSQSNPSTP VAQAQLTLLQ AEAVARKAKV NELELSQLSA NNRQELSRLQ VELYKKREAR VQAQLQSLRN NLNNQRQQAA EQALERTELL AEQGGDLPES ITQQLQRNRE LSQALNQQVQ RIDLISSQQR QAVAQTQQVR QALNTIREQA QWLGVSTALG ETLRAQVARL PEMPKSQQLD RDMAQLRVQR LQYEDMLEKL QQQKTTLKQD DGTPLTPDQQ RILDAQWRTQ SELLNSLLSG YDTQILELTK LKVANSQLVD ALNGVREATH RYLFWVADVS PISLSYPLAV AQDLTRLLSL DTLSQLSGAF VMMMTNQETL LPIIAALLFV GFSVSSRRHY HAFLERASSR VGKVTQDHFS LTLRTVFWSI LMALPLPVLW AALGYGLQNA WSYPMAIAIG NAVTATVPVL WVFMISASFA HPHGLFITHF RWSPTQVARA MRFYRLSVWL IVPLMMALIT FENYNDREFA GTLGRLCFIL LCIALSLVTN SLKRAGIPLY LDKKGSGENM INVALWGLLL SAPLIAALAS ALGYLTTSQA LLARLETSVA IWFFLLVVYH IIRHWMLIQR RRIAFDRAKQ RRADILAQRA RGEEDSPHSN STEGSIDVEE PIIDLDVISA QSLRLVRSIL TMIALVSVIV LWSEIHSAFG FLENIRLWDV SSTINGIESV QPITMGSLLI AVLVLIITAQ LVRNLPALLE LALLQHLDLT PGTGYAISTI TKYLVILFGG LLGFSLLGIE WAKFQWLVAA LGLGLGFGLQ EIFANFVSGL IILFEKPIRI GDTVTIRDLT GSVTKINTRA TTISDWDRKE IIVPNKAFIT EQFINWSLSD SITRVVLTVP APAETSSEEV TNVLMTAAQR CTLVLDTPPP EVYLVDLQQG IQIFELRIYA AEMGHRMPLR HEIHQLILAG YREHGITLPF PPFQARLETL GRGTGRTISS GRSRTPGSL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTAAGGAGCTCTTACGTG  

PCR Backward Primer

TTATCCACTTCCAATGTTACAAACTACCCGGTGTGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008