Clone

Target

IDP00199  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

590  

Reference Sequence Used

MTIASITLTN NRLEWQSLLP NTTPYEALFA TASQLEPVAF SAYQPRLENG MTLFCHPQSQ PRFMLIKAQE STDYLALIAQ AVKALQPEAS NLLGGDYLVH GHHVTWQSAQ HGDERFAAQS TCLYQEWVEP EQLFGCVRMY KDQITLQPGL VHQVNGGVLI LSVRALQAQP LMWLRLKQMI VQQRFDWLST DETRPLPVHI PSMPLDLRLI LVGDRLGLAD FHDMEPELGD LAIYGEFEAE LPLVDIEGMA LWCGYINTLL QQKQLPALSA DAWPVLFRQA VRYSGDQGSL PLCPQWLTQQ LAEAALYAEN DTISANALEA ALNARNWRQN YLAERMQDEI ELGQILVETE GHVVGQINAL SVLEYPGHPH AFGEPARISC VVHLGDGEFV DVERKAELGG NIHAKGMMIM QAFLMSELEL DQPLPFSASI VFEQSYGEVD GDSASLAELC ALVSALSQQP INQQIAVTGS VDQFGNVQPI GGVNEKIEGF FETCQRRGLT GNQGVILPTT NVRHLCLNQA VVEAVQKGQF HLWAVDTVAE ALPLLTGVTY DDEQQPSLLG AIQERIAQIN PQDKRRWPWP LRWLNWFNQG  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACGATTGCATCTATCACTT  

PCR Backward Primer

TTATCCACTTCCAATGTTAGCCCTGGTTGAACCAGT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008