Clone

Target

IDP00180  

Clone Suffix

4

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

856  

Reference Sequence Used

MRVIFMRQVT KSKKIKILPY PEWLVKAGMS KGIDHDRQHL GIILAAGEMI KVRQVNAEYK EKLKLYLLND NKNTQRSISF NTDWIELSVD AISVPFINTP YSDGIIPEIV FEYPDTSKLL PVYEKGEDES IFFESWDKQN AEFGVVESEY VIILIPEVSK DRLKSFSTSG GIDTVLGFYQ DIFSFNNSLA GLSFEPQRYS DGNTRNRYFA KADKGGGGAA YYSNNWIASS SGSINTFWLS PNATNWGCLH EIAHGYQGGF IDDKYFSTRE VWNNIYAACY QDVMLGAEKF NKGWLYNFGK QKEVEKSILN NINNGKEVNA WGERSKLYFI MLMLEKAGVN CFTHFNQIYR ERKNTDQSVG SVLDMLSNSF ATVENRVDVT LFLQMVGGHI SKNQYQRNLF SHAKAVYPLN QLLQGDELTA VKKTLDLNSE LSLVDVDSIE FTDIKSNLTL QFSIDDFAQI YGEELLILNG DNYVYNQSIM DKKQTLYNLP VGAYTLRIPT GRNKKYAPQI NYLIVKNEDS QTQVDFVHRI GSPIVSQKIS LQGLNDYTFA TIFFDQENDT VSVDITKTKP HYHFPGMYAR IRIKDKDNNE LLNEVITGTN QSLSKNDFPL SSCYVIDIFH KEPGRVKLTP AYKGVIDNKS SYNEFIITPY GLQNIKLNND PKVFLLENIK SAAEMLRSHP IMWHANFSEA KDNIYLAIDI FPSPQKEGLL EQYADCISSY YEKPNEDLGN AFSFIFKGIN DREFLTTKLN LVTKKLEVKI VSGTPHSGFK RTYAVLRYFN ADGNELLNLD IIGSKKQAGQ EWVFPISGYG GEKLYLQHVE PKNRLVITNI MQGIRLSSRT SIQTYEIESL GLSRCT  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAGGGTTATATTTATGAGACA  

PCR Backward Primer

TTATCCACTTCCAATGTCAGGTGCAACGACTCAACC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008