Clone

Target

IDP00030  

Clone Suffix

1 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

401  

Reference Sequence Used

MIHTLVRGQK IDVTNNHLEI RGLLVDLTWN APMNMDVDAS AFLVGFNGKI TKEEDFVFYG QPYSSCRSVQ LNQNETNGSK QRFSIDFTHI KDEVQKIVFS ITIHNAEEKK QALRDVSHIQ LKISNAQSGL EIIHFPITYP FTDESAIIVG ELYRHGGGWK FNPIGAGYFG GLAALCTSFG IEIADDEKQT APPIEKKIVP TPPPVQKAIN AVKVELKKKQ SINIQKSKMV TATLEWETNK DLDLYCFYVT TNGEIGKVYY KNLGSSKISP YIVLDGDSQE PGKETIRIYR PEALKYVLFA AYSAVGNGIG SFYSMKAKAV VDNHMGSVVT APLLEINDHA YWVCIAHIDF TNLNEMKISH VESYSKNHSE ASPLLYENGK FRMDVGPIEF KNEEDYQKYF K  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MIHTLVRGQK IDVTNNHLEI RGLLVDLTWN APMNMDVDAS AFLVGFNGKI TKEEDFVFYG QPYSSCRSVQ LNQNETNGSK QRFSIDFTHI KDEVQKIVFS ITIHNAEEKK QALRDVSHIQ LKISNAQSGL EIIHFPITYP FTDESAIIVG ELYRHGGGWK FNPIGAGYFG GLAALCTSFG IEIADDEKQT APPIEKKIVP TPPPVQKAIN AVKVELKKKQ SINIQKSKMV TATLEWETNK DLDLYCFYVT TNGEIGKVYY KNLGSSKISP YIVLDGDSQE PGKETIRIYR PEALKYVLFA AYSAVGNGIG SFYSMKAKAV VDNHMGSVVT APLLEINDHA YWVCIAHIDF TNLNEMKISH VESYSKNHSE ASPLLYENGK FRMDVGPIEF KNEEDYQKYF K  

PCR Forward Primer

TACTTCCAATCCAATGCGATGATACATACTTTAGTAAGGGG  

PCR Backward Primer

TTATCCACTTCCAATGCTATTTAAAATACTTCTGATAATCC  

Shipped To Lab

ANL  

Shipped Date

May 08, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008