Clone

Target

IDP00064  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

243  

Reference Sequence Used

MAMKVKEVAN LVGISVRTLH HYDEIGLLTP DETTESGYRL YSNENLEMLQ QILFFKELGF PLKKIKEIIM SPSFDRKEAL QLHKKMLLEK RARLDKVIAT IDKTIQHTKG EIEMTNKEKF KGFDFSHNPY EEEARERWGD AAVDKANEYA KGMSKEKQEE FNAIYRNLAA LRHGAPDSKE AQEAIKVWYD YLQNFSHYSL DAFKGLGQMY VADERFTKNI DKFGEGLAQF MCDAMEVYAD RNK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCAATGAAGGTAAAAGAAG  

PCR Backward Primer

TTATCCACTTCCAATGCTATTTATTACGATCCGCATAAA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008