Clone

Target

IDP00148  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Jun 06, 2011  

Experiment End Date

Jun 07, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

333  

Reference Sequence Used

MVLIIVRTLL MRISIKMIPL AYLNHFRKEH MKSRIHWAAL GLLAAFAAQA ETVTIEHRLG KTTLEQKPQR VVVIGVGALD AIDSFGIEPV AVSKFDGTPD YLAKYKSDKY PSAGSLFEPD FETIYTQKPD LIVIGPRASK SYDELSKIAP TIVFAAEADQ GYWESTQQQW RNLGKVFAIE PAVEAKIEQV DAQFKSIMQY NQQHKSDAML VMSSGGNLTT FGANSRFSSV YKDFGFSETV PVSKESSHGD LISFEYIREH NPKTLLVVDR DKVVTKGETN IRQTFENDLV KATTAYKNGH IAYLDVNAWY IAISGVKATE QMVADMKASV GMQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGGTTTTGATTATAGTACGCAC  

PCR Backward Primer

GGTTCTCCCCACGCTGCATACCAACTGACGCTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011