Clone

Target

IDP00401  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

2531  

Reference Sequence Used

MNKNLYRIIF NKVRGMMIVV ADIAASGRAS SSPSSGLGHT QHRRISALST LSFSLLLALG CVSLSVQAAI VADASAPGNQ QPTIINSANG TPQVNIQAPS SGGVSRNVYS QFDVDGRGVI LNNGHGVNQT ELGGFIDGNP WLARGEASII LNEVNSRDPS KLNGYIEVAG RKAQVVIANS AGITCEGCGF INANRVTLTT GQAQLNNGQL TGYDVERGDI VIQGTGMDSS RQDHTDLIAR SVKVNAGIWA NELSVTTGRN QVDAAHQNIN AKAADGSPRP TVAVDVAHLG GMYAGKIRLI GTESGVGVHN AGEIGASAGD ITITADGMLM NSGQINSSQQ LVVNTAADIE NTGVLYAQGN TQLTTAGTLS NSGTLAAGGD TSVRAAEVNS TRNSVLGAGV KSDNSAITSG TLSVEASGKI TAQGKNISGT AQRFTAHRLD LSGSQTQSRD ITLIAQGGEI DLTGAELLAS DRLSAATTAL LRTDNASLIA EQITLDAQAL SNVGGLIAHT GTTDFNLNLP GDVDNRGGTL LSSGTLSLQA ESLNSNGNSL LGAGVQSDGR LTEIGDLRVT TRQDLIAHGQ TLAAGAMALT GSRIDLADSY TQAREMTLTA NRGDISTQRA TVLALDTLSI NTAQTLNNQG GTLAGNTLAL DLGQFDNQGG QVTASQDLTI DLQRDFSHQA GSTLQAGRDL TLTSLGAVTN DGQLVAGGTL STHSDSLLNS GNLIATQAEL NATGALINHG EILTLGGLDT DSNTLFNTGS IISAEATLNA RERITNSGPD ALIGATDENG TLALLAPVIE NSDTVTHTDT APTTTILGMG TVILAGGHAR DGHYASAAQV LNLSGLIESG KDMLIYATTL TNSRHILTAN TDFIVADTVT GTAVWTAENP DIPGGRYAEP PDGGADNSDY IGTEYTSVIA YNGIDQISPE AQLLAGGNLT PQVGTLENFW SKVSAQGEID LTGVTLQQDG WGDQQRLMEQ TTSSGVWRYR TYKGGLWTRE WGPEVSERAT SEYASSFTAK TLSGSGTTIN NGANPGAIAP PADRDNSGKD LAVEFNGISL TQPNGGLYQF TTDHTVGGGG YLIETHPAFA NLNNWRGSDY VLQQLNNDPD VIFKRLGDNA YEQRLVRDQV LALTGQAVAS DYRSAQEQFE ALFAAGLEYS KAFNIALGTH LSAEQMAALT HNIVLMETRD VAGQTVLVPV VYLAGVKPGD LQANGALIAA ENISLTEVQG FTNAGAITAT NDLKISMAQD ITLNNRGGLL QAGGDMQLST LNSDIDLTSA RINATNLQLD SGRDVILRTD SAQLSSDNGA VSRDQTILGP LASINVSNNA TINTGRDFIM QGASLNVGQD LQVTTGGDWQ LETVQTRDQI STHDGRGSAT SEHIRHLGSE VNVGGALTAN VDNLTAVGAN INAATLEVQA QNISLSAATD SLHVTGESSS KRHTSSVNLY DETLLGSQLN ATGDINLQAA QDITLRASAV QTDGALTLAA GGDVLLTTQT EQHDEQRNHT GLSKGIASST LTRTEDSLSQ TLAVGSMLSA GSIDVSGKNI AVMGSNVVAD QDISLRAQEN ITVGTAQQSE SESHLFEQKK SGLMSTGGIG VTVGSSSTKM TDSGQSISSV GSTVGSVLGN VSMTAGEDLR VQGAEVLAGK DINLTGKNVS ILAAENQLTQ SHTVEQKQSG LTLALSGAVG SAVNTAVTTA KAASEESSGR LGALQGVKAA LNGVQAVQAG QLVQAEGGDA ASMFGISASL GSQKSSSEQH QEQTHVTGST LTAGNNLTIN ATGEGNAANS GDIVVQGSQL QAGGDTTLDA ARDVLLLGAA NTQKTDGSNS SSGGSVGVSL GISGASSGLS IFANANKGQG SEHGDGTSWT ETTLDSGGTL SLYSGRDTSL VGAQVSGETV KVEVGRDLLL QSQQDSDNYD AKQQNSSVGG SFSPGSMTGS ISINGSQDKL HSNFDSVQEQ TGIFAGSGGF DITVGGHTQL DGAVIGSTAT ADKNTLDTGT LGFSDIDNQA DFKVEHQSVG ISTGGNIGSQ FVGNMANGLL VGANNEGHAD STTHAAVSEG TITVRDTDNQ QQNVDDLSRD VEQANNALSP IFDKEKEQNR LKEAQLIGEI GSQVGDVFRT QGQIIATQAA TEKMQEVSEA DREAAKANWE KANPGQIATA EDINGQVYKT AYDQAFNASG YGTGGKFQQA VQAATAALQG LAGGDIAKAI AGGSAPYLAE VIKQSTGDNE EARLAAHAVV GSVLAHLQGN SAVAGGAGAL TGEIAADLIM QQLYPGKMVS ELSETEKQTI SALSTLAAGL AGGLTGDSSA DAVAGAQAGK NAVENNALGS LGDIFGSQGA KYFEGAGSLE RELSTDNTLT VQEKQAIRDH YLKGDLPEDV VKAILENTPA SDTVMALLQA ESTKDYALAL LSSLPLERAI AVLGKTANTL IKSSVVDKIL DAQRVGSGLK PDPSHRGASY LNREQLMAGE VFNITGGDGV KRSLLQTKGT FNGKDGVFEY IYDKTGNVTH QRFIEGVGIT GIPNQKAPKV K  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATAAGAACCTGTACCGTA  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTCACCTTCGGTGCTTTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011