Clone

Target

IDP00506  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

562  

Reference Sequence Used

MRYCYFILHT AILFPLLVIS RAGQSAELTP IQQQTLHQQE RQRALEERLA PPTPDVRLSA PSASSDRLIF PVEKPCFVID RVTLSGTEPL PRWLPLQRIA NQALGQCLGG QGINQLMSQL QNRLVGHGYV TTRVLAPQQD LNSGTLALQV VPGKIHRVAL TPESDRHVTL FSAFPARPGH LLDLRDIEQG LENLQRIPTV QASMELIPGS APGETDIALS WKQSKMWRLA ASLDDSGTRS TGRYQGGATL FLDNPFSLSD QFYVSAGGSI KNRGDKGTNN LTGHYSLPFG YWTAGITASS YDYHQTVAGL NSDYNYRGES ENVSLQLSRL LHRNASQKTT LTYDVLTRSS KNYINDTEVE VQRRRTSAWR IGLQHRHFIS QATLDAGVSY QRGTRWFGAV PAQEEYFDEG TALSKILQLN AQLDIPFSVM AQNFHYNVQY QRQSSSTPLT PQDQFSIGGR WSVRGFNGER TLSADRGWTV RNDLGWYTPI QGQEIYVGLD YGEVGGRGSA YLLGNHLAGA AVGIRGNVLN TRYDLFAGKP LSEPDGFQSD AVTVGFNLNW QY  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCGTTATTGTTATTTTATATTAC  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTATTGCCAGTTCAGGTTA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011