Clone

Target

IDP00168  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

2417  

Reference Sequence Used

MGLKMKEMTA MQAAYWLGRQ HDCLLDGVAA HLYAEFDGQA LNRQALTEAV RALYAKHPML RLAITKDGQQ KILPLSTFHQ LKVDDLSQWK PDEVESFVHT KRQRMTHQML DLTQGNPIEI SLTLLPEGKH RLHIDADMIA CDAQSFRLLV DDLTSLYLEA IEHRLEIIES DVVTFFQYLD AQQADRALAK RKEVDKKWWQ ERLATIPAEP SLPYQPVPTD AVSANSQRFA HWFTPVERKG LAEVARQHHL TLTQLTLALF SQVIANACQE RQFRLNVPTF HRGNRSLDIE HTIGDFSNLL IFSADVGTTQ TLLSLCQQTA NQLHQLLRHE SYSGVSVMRD LSRKQGGVQR SPIVFTSGME MRDEEIFSDH VTQHLGRMNW VISQGAQVTL DAQIAPAYEG ILLNWDVRME NFADKDITAL FAHYVDLIRC VALHPEMMQQ SVQQIDAQLG YARRESIQEM PLTPLQQAYL LGRSTQIALG GVAMHEFREY RGHIDTQSLH SRLLYLVEHI PALRTRIDQE KWIQWVSPCI ALNWQAIDLQ HLSREQALLA VEPVRQQYQQ RMHDLTRSPW QICVVQLPIE EQEEFSSIVL TSFDALIVDG RTHALILAAL LGSEEPDITQ VVQNARDTQS ISPQFASKKA QDEAYWKSKL HPDCPPPALP WKQALETITT SRYARESLQI PKESVGKLNR CGIENGLFLN SLLTATILDV LSYWTTEIGM RVGFPVLIPS SNAIDGNESS FVILEHEKST LSLLSQASKL QREMLEALEH LAFSGVDLNR LLMNQAPQAL VLPVVLTNGL SWKTLNPEDA VTLFDGVTQT PQWL*IFA*L TTSRKTSSSA LIML*RF*RL SLSARC*VPY IIA*VRSLRQ HHWPRRLSHV STFHTTVLIA MSRPVTIAIF LPS*RSNCLS AQRIKPL*SA VNKPFLMLNW VSRFSA*CGS SKREG*PQEM CSPFACHAVL NISSSVWLLH FRGLSGYLLM LRLPKSV*IT F*KIVTPIWW *WISPVSLAM *SLLMP*LSL SYSPMEYLTL HHSIN*AD*A KANKRLIIFI PLALRANLSA WWSIIRRPRM *LVKLAKRGI SPVRMW*CQ* RHCIMICRCL ISLPP*ASER R*FFPLGMKK KMRCNGIA*L SGIK*RFGSP SPLSWRCCSL ALKQGNYTHF V*LPKGGITS NLPPSPNYAL VQIRRG*SLW AVLPRRRFGA SGMS*RQMMS A*SHMVGRWR ETVISLWMRY NAMYRKVWSA VSSPLGSI*R KVILKTAN*N KPILSPYWMS MATLCARFER AIKATTARTA ISFLLAASMV M*KFVGYECH CRISKSSCKL IRRSRAWWWS IMRILMEIPL *PHYLALNLS NQHRVRRYES LPNNRYRLRI FRVGLLL*RL YRFLRMVKWI ANSAKRMFSD NRSLLNQ*VN RIKVNLSHHL PCHRLP*VSL NSESGDSIND MVTDAINMRP LID*AEK*IS RA*SQRSVSC PIIFQC*TDA MCLMRHPA*P CTRRSQHRLR SILSMLSQWM RRLNILCDGK ISRWI*PSKP H*VFAC*VWG AKSECLG**A IKLSASNGIG GAYLSA*PMG ITKSPVTSIL *WRGRIFPSA LPL*CRKRHY PKRCCRGYSL RATLHGLSRA CRAQIAPSIR FAVVNIGLPY TAAYWRSMV* RSRISRKCLH *SPRYLLATL PQVRNLANLS FTFRMMWSAK RVS*MVL*LK ASWCISPLKA LSVRFRN*AK RS*TLCASR* RGESLAKLTP LPQPW*LG*W ILQCIYNWRA CAAKNYFLQP CTPNLKSPWG LGLIAKVP*Y LSWR*MPRFP PMSEPTCLSN LSLR*VGEQC LLRQPHPLMS QPLS*RQTIR RPQTMRMRRW QSLSLN*AL* IPLRLKSAFW LSFAQHLVLP R*RQRMISLI LADIP*SQRE SLVAC*ASKV LNCTSMICLV SLMPSNWRSK PYFTVNPPAH HSQ*VRWSSR PKRLCR*HKP LYGRR*ANTP SLV*PTSSIF RLRLNF*MRS MSKHLVRLFT GFYCVMLGYE PTLA*KMGSL TST*SQPAT* NITSGSGRLK ITPPNPWPDY WRKRPSTPLT *AKNCHFDSI SYVMNRRGRN IFHFCSTILC WMSGRSIF*W MN*HRCINTV CKVLVHSGKR NPYLSMSLLA SSVLRHSIKR ILTIG*PSLP VCLGHSLCLL RIIL*VTALA SILEKAAGSK *NCRNQPWYR CINWLKHAML RCLT*CMRRF LRRYIVSAHQ KSCWLAHLLP VGWMPSFSTQ SVISLPWAFS WWISRKYKRC GNSSSK*KTA LTSRCHTQIF RLT*LKKGSK VLSMRRKVTC LRSLYNCMQK ISCMESYC*Q KGMRSASNKS IPIKVNPA*V YSLKF*KRGS SKSRR*ES** VICRNTIAQL KSLC*PR*PV ACLSGFPIVL HKILHCRR*K SR*GSLKMKL VALRPWG  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGGTTTAAAGATGAAAGAAATG  

PCR Backward Primer

TTATCCACTTCCAATGCTACCCCATGGACGGAGAG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008