Clone

Target

IDP01010  

Clone Suffix

5

Sequence Comments

 

Experiment Start Date

Jun 06, 2011  

Experiment End Date

Jun 07, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

616  

Reference Sequence Used

MALLQISEPG LSAAPHQRRL AAGIDLGTTN SLVATVRSGQ AETLPDHEGR HLLPSVVHYQ QQGHTVGYAA RDNAAQDTAN TISSVKRMMG RSLADIQTRY PHLPYRFKAS VNGLPMIDTA AGLLNPVRVS ADILKALAAR ASESLSGELD GVVITVPAYF DDAQRQGTKD AARLAGLHVL RLLNEPTAAA IAYGLDSGKE GVIAVYDLGG GTFDISILRL SRGVFEVLAT GGDSALGGDD FDHLLADYIR EQAGIADRSD NRVQRELLDA AIAAKIALSD ADTVRVNVAG WQGEITREQF NDLISALVKR TLLACRRALK DAGVEPQDVL EVVMVGGSTR VPLVRERVGE FFGRTPLTAI DPDKVVAIGA AIQADILVGN KPDSEMLLLD VIPLSLGLET MGGLVEKVIP RNTTIPVARA QDFTTFKDGQ TAMSIHVMQG ERELVQDCRS LARFALRGIP PLPAGGAHIR VTFQVDADGL LSVTAMEKST GVEASIQVKP SYGLTDGEIA SMIKDSMSFA EQDVKARMLA EQKVEAARVL ESLTGALTAD AALLSAAERQ CIDDAAAHLS AVAQGDDVDA IEQAIKNVDK QTQEFAARRM DQSVRRALKG HSVDEV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGGCCTTATTACAAATTAGTGA  

PCR Backward Primer

GGTTCTCCCCACGAACCTCGTCTACGGAATGGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011