Clone

Target

IDP01435  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Jun 06, 2011  

Experiment End Date

Jun 07, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

323  

Reference Sequence Used

MKKLLSIVLG LALSAPVLAN EVSFEQYVER LKQQAREEGI SERILNEAFR DVEYKPRSVV ADKNQPEKKL TLDEYIPRAV PEWKVKQAQS LYEKHYTELQ RIGKQYGVQP RFIVALWGVE SNFGAFTGNF RVIDALSTLA YEGRREEFFR KETMAALQIL EQGHIAPEAM KGSWAGAMGQ CQFMPSSFLN FAADGNGDGK KDIWGTRSDV FASTANYLSQ SGWDDKYTWG RQVKIPKGFD HALEGRQPEK GKTLQEWSKL GVTRYDGKPL PALSDDIKAW LIMPDDEAGR IYLVYNNYNV LMKWNRSHYF ALAVSHLADR IAF  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGAAAAAATTATTATCGATAGTAC  

PCR Backward Primer

GGTTCTCCCCACGGAACGCAATCCGATCCGCC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011