Clone

Target

IDP00150  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

559  

Reference Sequence Used

MRKIVLASVV TSLVGCSMGH RDPVEAKQAL NQAINETNSR QIDQLPPSVE ADLMPDMDTL TASEPKTLQR FRIQAEDVEA KAFFASLVQG TEYSAAIHPA VTGRITLNLT DVTLDEALGV VRDLYGFEVV KEGKVIQVYP AGLRTVTIPV DYLQFKRTGR SLTSITTGTI TNTDTNNSNS SSSSSSSISS NSSSDGSSSN SNSNRSDARG GTEIETTNES DFWPLLEKAV AQLLGGSGGQ TVIVNPQAGV LTLRAYPDEI RQVNEFLGIS QQRMHRQVIL EAKILEVTLS DGYQQGINWS KAFSSNGANY KIGSGSITQD SNGNPITSVL PGLDAIGNLL GGQSNVVISS GSFDAVISFM ATQGDLNVLS SPRVTASNNQ KAVIKVGTDE YYVTDLSSVV GTGDNAQASP DITLTPFFSG ISLDVTPQID DQGNVLLHVH PAVIEVEQQT KKILYRSEEI ELPLARSSIR ESDSVIRAKD GDVVVIGGLM KSNTVDQVSK VPFLGDVPAL GHLFRNTTKL TQKTELVILL KPTVVGVNTW QKELERSRSL LQEWFPDSQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCGTAAAATCGTACTCGCT  

PCR Backward Primer

TTATCCACTTCCAATGTTATTGACTATCCGGGAACCA  

Shipped To Lab

UT  

Shipped Date

May 08, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008