Clone

Target

IDP01655  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2011  

Experiment End Date

Jun 07, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

741  

Reference Sequence Used

MLKKIVTALG MSGMLLASSN AIAEDTTTKN DNLSPQSVDL SPLRNLNKLD SPMDKDYNYH QAFKKLDTEQ LKKDMQDLLT QSQDWWPADF GNYGPFFIRL SWHDAGTYRI YDGRGGANRG QQRFSPLNSW PDNVNLDKAR QLLWPIKQKY GDAVSWSDLI VLAGTVSLES MGMKPIGFAF GREDDWQGDD TNWGLSPEEI MSSNVRDGKL APAYAATQMG LIYVNPEGPD GKPDIKGAAS EIRQAFRAMG MTDKETVALI AGGHTFGKTH GAVPEDKVKQ AIGPAPDKAP IEQQGLGWHN SYGTGNGDDT MGSGLEGSWT STPTFWNHDF LHNLYNLDWK KTLSPAGAHQ WTPTNAKPEN MVPDAHKPGV KHKPIMFTTD LALKEDDGFN KYTQEFYNNP EEFKEEFAKA WFKLTHRDMG PKSRYIGPWI PEQNFIWQDP VPAADYKQVS TQDIAQLEQD IINSGLTNQQ LIKTAWDSAS TYRKTDYRGG SNGARIALAP EKDWQMNEPA KLEVVLTKLK EIQTNFNNSK TDGTKVSLAD LIVLGGNVGV EQAAKQAGYN IQMPFVPGRT DATQAQTDIE SFNYLKTKSD GFINYTDGSV SADKLPQTLV EKASMLDLNI PEMTVLVGGM RALDVNYDNS QEGVLTTTPG QLNNSFFVNL LDMSTQWKKS DKKDGEYIGI DRKTGKQKWT ASPVDLIFGS NSELKAVAQV YAENGNEQKF VNDFAKAWHK VMMLGRFDVQ Q  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGCTAAAGAAAATTGTAACTGC  

PCR Backward Primer

GGTTCTCCCCACGTTGTTGAACATCAAATCTGCCA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011