Clone

Target

IDP02374  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2011  

Experiment End Date

Jun 07, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

868  

Reference Sequence Used

MSSFVLDFQE IEKTQLSLVG GKGLNLGALS NMQGIQVPEG FCVTTAGYEK AIEQNEELQA LLQQLTKLKR EERVRIGEIS KKIRENIMAV QIPSDVVEAV AHYLSRFGNE HAYAVRSSAT AEDLPYASFA GQQDTYLNII GENAILQHVR KCWASLFTDR AVMYRMQNGF EHNQVSICVV VQKMVFPQAS GILFTADPIT SNRKVLSIDA SFGLGEALVS GLVSADNYKV KEGEIIDKMI ATKKLAIYAL KEGGTETKQN DPVQQKIQTL SDQQILQLVQ IGRQIEAYFG CPQDIEWCLV DHTFYIVQSR PITTLYPIPE ENDEGNHVYI SVGHQQMMTD AMKPLGLSFF LLTTNAPMRK AGGRLFVDAT QQLVLPASRD YLINTLGKSD PLVRDALTTI IERDDFITLL PDEEKEKSAS KSKPPVSSQP EIENDPAIVT ELIRNSEASL EELKQNMQLK SGVDVFDFIL EDIQQLKKVL FNPQSIAVIM AGMNASTWIN EKMEQWLGEK NAADMLSQSV QNNITSEMGL ALMDVADAIR PYPEVITYLQ HVEDDSFLDE LVQYKGGEKA RDTIVAFLNK YGMRCSGEID ITKTRWSEKP TTIIPMILNN IRDFEAGASK QKFEKGLQEA LNKEEELLDR LQHLPKGKQK VEETKRMIRN IRNFIGYREY PKYGMINRYF IYKQALLKEA EQLVQSGVIC EVDDIYYLTF EELHEVVRTN KLDYELIQKQ KNDYKLYEKL TPPRVMTSDG EIITGKYKRE NLPADAIVGL PVSSGVVEGR ARVILNMEEA NLEEGDILVT AFTDPGWTPL FVSIKGLVTE VGGLMTHGAV IAREYGLPAV VGVENATKRI KDGQRIRVHG TEGYIEML  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGAGTTCTTTCGTTCTCGATT  

PCR Backward Primer

GGTTCTCCCCACGCAACATTTCAATATACCCTTCTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011