Clone

Target

IDP02473  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2011  

Experiment End Date

Jun 07, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

463  

Reference Sequence Used

MHLHFTPRQI VEKLDQYIIG QKDAKKAVAV ALRNRYRRSK LAENLRDEIA PKNILMIGPT GVGKTEVARR MAKLVGAPFI KVEATKFTEV GYVGRDVESM VRDLVETSVR IVKEEMVVKV QDKAEEQANQ RLVEILVPSP EKQSGFKNPL EMLFGGTQNS NQTTDSQEDV EIEKKRQDVE RKLAAGLLED EIVSIEVTEQ QSSMFDMLQG TGMEQMGMNF QDALGSFMPK KTKKRKLSVK EARKVLTNEE AQRLIDMDEV TQEAVYRAEQ LGIIFIDEID KIAGKQSNSV DVSREGVQRD ILPIVEGSNV ATKYGSVKTD YILFVAAGAF HMSKPSDLIP ELQGRFPIRV ELTKLSTDDF VKILIEPDNA LIKQYMALLA TEGIEIEFSD EAIRKIAEIA YQVNQDTDNI GARRLHTIME KLLEDLSFEA SEITLEKITI TPQYVEEKLA TIAKNKDVSQ FIL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGCATTTACATTTTACTCCGC  

PCR Backward Primer

GGTTCTCCCCACGCAAAATAAACTGGCTCACATCT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011