Clone

Target

IDP04004  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

266  

Reference Sequence Used

MNILKKTTSV LLAILITSFS LIACESKKNV EASKEENTIG IIGAMNEELE VLLKDMKNQK EVKRNDLTFY EGDLWGQHVV AVVSGVGKVN AASCTQILAS EFNVKSLINI GVAGGVSKDI YPGDIVIGDT YVQHDVDASV FGDKIGQIPR MDVYDFKADE KLLNLAKEAA KNVPEVKTYV GRIVSGDQFI ADSNKVKVLD EEFNAKAVEM ESAAIAQVAY LNKIPFVIIR SISDNANNGA HMDYKEFIPV GVKNSTSILK SMFENM  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAATATATTAAAAAAGACAACAAG  

PCR Backward Primer

TTATCCACTTCCAATGTTACATATTTTCAAACATTGATTTTA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011