Clone

Target

IDP04012  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 21, 2011  

Experiment End Date

Jul 21, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

33  

Sequence End

176  

Reference Sequence Used

YHENKIKNEM DSLYKSSYSI LRLYFQVNNL EGNKGNFNRY IPKISNDISE QGKCQIDVFN EDLEKEYSYY LDDFSFDYEK DGGYDVLKEA NKNKAALNIY RDENAVKAYL AFPVYVDESF LGNILLSKDY SKEYLELRDL MSNI  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGTATCATGAAAATAAAATTAAAAATGAA  

PCR Backward Primer

TTATCCACTTCCAATGTCATATATTGCTCATTAAATCTCT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011