Clone

Target

IDP04013  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

498  

Reference Sequence Used

MTSLPTPIIG LIVAVFVLVW LVLRTRVHAL IAMLAAACIA GLLGGMGIDK TLSVITSGFG TTLGSIGLVI GLGVMMGRLL EVSGAAERIA WSFIKWLGKR REEWALAITG YIVSIPIFVD SAFVILYPVA KALAKSGKRS LLTLGVALAG GLVVTHHTVP PTPGPLGVAG IFNVDIGAML LTGMALAVPC VIGIVFYAQW LDKRYPDFVP RTLNADEVNA ALEQYNKEKE QKELPSLTLS LLPIVVPIVL IFLKAICSTL ATVEGWSGLA THPVVQAINF VGSPVIALAI SVLLAVYTLV PRMDKHTTAE RLEEGLQSAG IILLVTGAGG ALGAILRDSG AGQQLAEQVA NLPISPILIP FIVATLVRLI QGSGTVAMIT AASISAPILA QIPGINMLLA AQAATMGSLF FGYFNDSLFW VVNRMMGVSD VKQQMVVWSV PTTIAWAIGG TGVVLINLLF GSGGSWLDPL LPIVVLAAIM LWVRWQAQGI KDKLVVKD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACAAGTTTACCGACCCC  

PCR Backward Primer

TTATCCACTTCCAATGTTAATCCTTAACTACCAGCTTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011