Clone

Target

IDP00161  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

503  

Reference Sequence Used

MSIDIKYLSR IDIDREEFFF KDSRLMCKKF DEEREVLTLL EFDTKFRVNL LKKDKVYKYF LVSDANHKLL IANLVTEQQA KDLSFIEKDI MKIASSATAY GASDIHFIRE DRICKIKFRV NGTMIDYREI LSSEADALMF VLYNVMATTK ETTWNRKLPQ DANIILVINE KAYRFRYAHM PLFGEGGKNY HAVVRIIYPS NNFVCTNYQD IGYNEADTDA IARILNTSYG LFIVSGTTGS GKSTSLKKYI ELLFFNKYKG KGCFVTVEDP VEYLISGAQQ SSIVADNDDK TKNPFADAVR SAMRRDPDVI MIGEIRDKPT VEALSSAVES GHYCLTTIHA GSVVSVLQRL SGLGMKADKI ASPGFLAGIT SQKLIPELCP SCKVSFVDER YQRAVFSANE NGCEACNHSG FKGRLLLLET LVPTVEDLEL VASENWVSLY RKYRERRFIK TGKKGLGEGF SIKDKAYYNV LKGKVCHEYF MLHFGQLDHE DENIIYENYL QEV  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTCAATTGATATAAAGTATTTAAG  

PCR Backward Primer

TTATCCACTTCCAATGCTATACTTCTTGGAGATAATTTTC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008