Clone

Target

IDP04162  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

405  

Reference Sequence Used

MLFVWQHKLV LVSCAQPAVF HSAKSLKRCL NSDRNVSMSL ADQVLAVNDD LPIRTHQPVH SGKVRSVYWL TEQDSRRLIK EKGYPVAEDA PLAIMVISDR ISAFDCIWHA EGGVHGVPGK GAALNAISNH WFKLFKQHGL AESHILDIPH PFVWIVQKAR PIKIEAICRQ YITGSMWRAY AKGEREFCGI TLPEGLEKDS QLPELLMTPS TKGILRGIPG VPEADDVNIS RQDIEANYAA FNFNQPQDID HYETLLKQGF AVISEALKSV GQLFVDTKFE FGYVADQQGQ QKLIYMDEVG TPDSSRIWDA QQYQQGKIVE NSKEGFRQFL LNYFPDPDIL LNKDRMPERE ALARDNALPL EALMDISRTY LGIAEKITGQ PIHLSHQPKQ EIIAILDKEY GLIER  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTTTTTGTCTGGCAGCAT  

PCR Backward Primer

TTATCCACTTCCAATGTTATCTTTCAATCAGCCCATATT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011