Clone

Target

IDP00164  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

256  

Reference Sequence Used

MYFMKSKNRF LLISLLSFST SVFADVNLYG PGGPHVPLIK VAESFEKSQS KRVNITFGPQ ATWNDKAKKN ADILFGASEH SALAIAEGHS ERFSKFNIHP VFMREAIILV KKGNPKNIKG MADLLKPGIG IVVNDGAGVS NTSGTAVWED SVGRMKNVEK LQAFRSNIHV FAPNSGSARK AFVDGEDIDA WITWVDWAIA NPTIGDMVRM EDEYRIYRDF NVVLAKNPSS EAIDFFDYLT KSKDAEAIFQ HYGWFK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTATTTCATGAAAAGTAAGAATC  

PCR Backward Primer

TTATCCACTTCCAATGTCACTTAAACCAGCCATAGTG  

Shipped To Lab

NU  

Shipped Date

May 08, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008