Clone

Target
IDP04258  
Clone Suffix
2 The clone is not available in BEIR.
Experiment Start Date
Jul 21, 2011  
Experiment End Date
Jul 21, 2011  
Person
Sofiya Shatsman  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
29  
Sequence End
159  
Reference Sequence Used
NHKYLEESKN VLNEYNKVIA LLLENDNGNI KSELERIESN NDMKNIRITY ISKDGNVIFD THKKLINDNE SYLKRQEIIE AIESGLGSSV RYSNDLHQNM IYSALKLKDG SIVRTSIAVE NAKILDSINS N  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
NHKYLEESKN VLNEYNKVIA LLLENDNGNI KSELERIESN NDMKNIRITY ISKDGNVIFD THKKLINDNE SYLKRQEIIE AIESGLGSSV RYSNDLHQNM IYSALKLKDG SIVRTSIAVE NAKILDSINS N  
PCR Forward Primer
TACTTCCAATCCAATGCGAATCATAAATATTTAGAGGAATCAA  
PCR Backward Primer
TTATCCACTTCCAATGTCAGTTACTATTTATGCTATCTAG  
Shipped To Lab
UT  
Shipped Date
Oct 11, 2011  
Secondary Shipped To Lab
UT  
Secondary Shipped Date
Oct 11, 2011  
Inserted
Oct 04, 2011  

Related Expressions

           
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2011-07-25 2011-08-02 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-10-04 2011-10-04 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2012-02-02 Maite Garcia University of Calgary Expression_protocol_Toronto success E. coli 15N 2X M9 none production
2012-05-06 Olena Onopriyenko University of Calgary Expression_protocol_Toronto success E. coli Se-Met none production
2012-04-20 Olena Onopriyenko University of Calgary Expression_protocol_Toronto success E. coli LB none production
2012-02-16 Olena Onopriyenko University of Calgary Expression_protocol_Toronto success E. coli LB none production