Clone

Target

IDP00147  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

261  

Reference Sequence Used

MCRYARSYLL HAFDYTLFVK ARLNMAFMIS MPAIELAGLT IQVCQFDPQQ FDEDQQHYLA PPTYHSLHKA VVKRQAEFVA GRKLAQQALK QIGQGYDRPI AIGTHREPLW PAGITGSIAH CDGWAVCTVL KAEHLSLGID IEHRLAHQTA SEVQAIIGTA QEWALLAQQF DLASAVTLLF SAKESLFKAL FPQVHLYLDF CDAQLSSLEP NKMSFTLTRV PNAAQQSCEV YFRWFEQQRV LTVSVLPMLG KIEYHVPTLP T  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTGTCGTTATGCCCGATC  

PCR Backward Primer

TTATCCACTTCCAATGTCAAGTAGGTAAGGTGGGGA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008