Clone

Target
IDP04512  
Clone Suffix
2 The clone is not available in BEIR.
Experiment Start Date
Jul 22, 2011  
Experiment End Date
Aug 01, 2011  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
256  
Reference Sequence Used
MRKKISLPII MAIGMLSLLF VGCTNNKEEK TLTVSIAASL QKPMEKIASN FEKENGVKIQ YNVGGSGTLK KQISDGADVD LFFSANTKYA KELIDEGLVN KDESYKILNN QLVLIENNDY NKPINSLEDL KNVEGKIAIG EISTVPAGQY AKESLEKVGA YKDIEDKLVF ARSVTNVKTY VENGEANLGF VYKTDALDLK NSKIVYEVPN DYHKPIEYEL CLLKNSKNNK EAKKLIEYLK GEESKKIFSE YGFEVN  
Reference Sequence Variance
MATCH  
Confirmed Protein Sequence
MRKKISLPII MAIGMLSLLF VGCTNNKEEK TLTVSIAASL QKPMEKIASN FEKENGVKIQ YNVGGSGTLK KQISDGADVD LFFSANTKYA KELIDEGLVN KDESYKILNN QLVLIENNDY NKPINSLEDL KNVEGKIAIG EISTVPAGQY AKESLEKVGA YKDIEDKLVF ARSVTNVKTY VENGEANLGF VYKTDALDLK NSKIVYEVPN DYHKPIEYEL CLLKNSKNNK EAKKLIEYLK GEESKKIFSE YGFEVN  
PCR Forward Primer
TACTTCCAATCCAATGCGATGCGCAAGAAAATAAGCCTAC  
PCR Backward Primer
TTATCCACTTCCAATGTTAGTTCACCTCAAAACCATAC  
Shipped To Lab
UT  
Shipped Date
Sep 27, 2011  
Secondary Shipped To Lab
UVA  
Secondary Shipped Date
Apr 25, 2017  
Inserted
Oct 04, 2011  

Related Expressions

       
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2012-02-23 Olena Onopriyenko University of Calgary Expression_protocol_Toronto success E. coli Se-Met none production
2011-08-02 2011-08-02 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2017-05-03 2017-05-05 Ewa Niedzialkowska University of Virginia uva_expression_native_1 success E. coli TB IPTG production
2017-07-09 2017-07-11 Beata Mrugala University of Virginia from other group success E.coli SEMET IPTG production