Clone

Target

IDP00231  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

331  

Reference Sequence Used

MELLCPAGNL PALKAAIDNG ADAVYIGLKD DTNARHFAGL NFTDKKLQEA VNYVHSRKRK LHIAINTFAH PDGYARWQRA VDMAAQLGAD ALILADLAML EYAAERYPQV ERHVSVQASA TNDEAIRFYQ RHFDVARVVL PRVLSMHQVK QLSRTSPVPL EVFAFGSLCI MAEGRCYLSS YLTGESPNTV GACSPARFVR WQQTPQGMES RLNDVLIDRY EDHENAGYPT LCKGRYRVDG QRYHALEEPT SLNTLELLPE LFAANIASVK IEGRQRSPAY VSQVAKVWRQ AIDLYQANPA QFAAKAEWME QLGAMSEGTQ TTLGAYHRKW Q  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGAGCTGCTGTGTCCTG  

PCR Backward Primer

TTATCCACTTCCAATGCTACTGCCACTTACGATGATA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008