Clone

Target

IDP00209  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

417  

Reference Sequence Used

MLGSLTVMTL AVAVWRPFIY HPEHEPVAKS VVLDTSQSRI LLPEASEPLD QPTPDDAIPQ DELDTKDAND TGVHEYVVST GDTLSSILTQ YGIDISDVSL LANQNRDLRN LKIGQQISWT VNDTGDLQSL TWEVSRRETR TYNRVGNNFK ETKELQKGEW KNSVLTGRLD GSFVGSAKKA GLTAAEIRAV TKALQWQLDF SKLRKGDQFA VLMSREMLDG RSEQSQLVGV RMRSGGKDYY AIRAEDGKFY DRQGSGLARG FLRFPTLKQF RVSSNFNPRR LNPVTGRIAP HKGVDFAMPV GTPVLAVGDG EVLISKFSGA AGNYVVIRHG RQYTTHYMHL KKLLVKPGQK VKRGDRIALS GNTGRSTGPH LHYEFWMNQQ AVNPLTAKLP RSEGLSGKDR SEYLAIVKQV IPQLQLD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTGGGGTCGTTGACTGT  

PCR Backward Primer

TTATCCACTTCCAATGCTAATCCAGTTGTAACTGCGG  

Shipped To Lab

NU  

Shipped Date

Jul 16, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008