Clone

Target
IDP00212  
Clone Suffix
3
Experiment Start Date
Apr 21, 2008  
Experiment End Date
Apr 22, 2008  
Person
Jason Stam  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
236  
Reference Sequence Used
MRDKTLKAIL AHAEAEHPKE CCGVVAQKSR VEKYFPCENL ATNPTEQFHL DPTGYVEAED WGTITAIVHS HPDATTQPSE LDMAQCDNNE LPWHIVSWPE GDLRTIQPRG DLPLVGREFV LGHTDCWGLI MSYFRQTHDI ELKDYRVDRH WWESGTENFY MDNWYECGFR EFSGPAQPGD LIIMQVSAPV ANHGGILLED GMLLHHPYGQ LSQRVPYGGY WQERTMKVVR YEISQL  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAGAGATAAAACGCTTAAAGC  
PCR Backward Primer
TTATCCACTTCCAATGTTATAATTGTGAAATTTCATACCTC  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2008-04-25 2008-04-26 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli M9 minimal 1mM IPTG assay
2008-04-25 2008-04-26 Jason Stam J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2010-05-24 2010-05-25 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay