Clone

Target
IDP04670  
Clone Suffix
1
Experiment Start Date
Jul 21, 2011  
Experiment End Date
Jul 21, 2011  
Person
Sofiya Shatsman  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
169  
Sequence End
401  
Reference Sequence Used
QTQVRLQNEL MNAEKFHLIG EMAASISHEI RNPLTSTKGF LQLLQSDTCT EQERKLYIDI AINGIEQANH VLTDYLTFAK PSIEKEQRLQ LEEELLHALS LITPLANLTN VRTHYIKQST SFFIAGEKQK LNQCLLNILK NCIEAMPKGG DLYFTLVPDH KHIQLYIKDT GVGMDQEQVK RLGSPFYSTK EKGTGLGMMV VFSVVQAMNG KIDIISEKGT GTTFLLTFPL IQK  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGCAAACTCAAGTTCGACTCCAA  
PCR Backward Primer
TTATCCACTTCCAATGTCATTTTTGTATGAGTGGAAAAG  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2011-07-25 2011-08-02 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-11-04 2011-11-04 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2011-10-04 2011-10-04 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay