Clone

Target

IDP90659  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2011  

Experiment End Date

Jun 07, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

384  

Reference Sequence Used

MKTILLAISG SIAFYKSYEL ISLFKKEGFR VKVLLSNGLL KFASKMSFEA LVDEVLCEEN ESWQNSNNHI AFSKDVDLVL FTPASVNSIN KLAFGIADNL FIQTLIAANK PLIIAPAANT NMFHHFSTQN SLKILKENKA LIIEPICKVL ACKDEGVGAL AEVKDIFNIT KRELLKEKFW CNKSVVITGG GTRERIDDVR CVSNFSSGKM AKAIADAFYF LGARVKLLSS VEFDTPYELC KFESSKDLKE LLDKNLSHDF LIMTAAVSDF IPQSVKGKIK KNEHLQGLNL HLSLNEDLLK TCKFQGKKIG FKMEFDSQNA LENAKKSLKD KQLDMVCLNI IDQKNYFGSD QNELYFITLN NENKSTLQSK EKLAFELVKW CEKL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGAAAACCATACTTTTAGCCAT  

PCR Backward Primer

GGTTCTCCCCACGTAATTTTTCACACCATTTTACTAAT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011