Clone

Target

IDP90741  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jun 06, 2011  

Experiment End Date

Jun 07, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

opMCSG28  

Sequence Start

1  

Sequence End

462  

Reference Sequence Used

MRLLDSVTKE KIKLDKKDIS IYLCGPTVYD DAHLGHARSS VCFDLLRRVL LAQGNRVKFA RNYTDIDDKI LKKMAQSGQT LEEITEFYIK SYEEDMRVLN VLDPDFKPRA THYITAMLDL IKKLAKDGFV YTLEDGIYFD TSKDEKYLSL SNRNLEENIS RLSNEVQKRN ESDFVLWKFD ENFYESEFGK GRPGWHTECV AMIDSIFENT LDIHAGGIDL LFPHHENEAA QCRCGCKRKL ANIWLHNGFV KIDGEKMSKS LNNSFFIKDA LKEFMGEALR FYLLSSHYRS HFNYSLSDLE NAKKRLDKFY RLKKRLDLGE ISDFDVLNDI GIKSEIAKQI LEILNDDLNV SKALALLDDF ISSANLELDK ESKNKILKQN IKEALSELAK IFGFGFMDAT LYFQWGVSKE EREEIEKLIL ERTEAKKNKD FNTADAIREQ LNSKKITLLD TPNGTIWEKI NA  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

GTCTCTCCCATGAGATTATTAGATAGTGTAACA  

PCR Backward Primer

GGTTCTCCCCACGTGCATTTATTTTCTCCCAAATTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011