Clone

Target

IDP90981  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

731  

Reference Sequence Used

MIIDIICFYI YAYIITIALR YKKTNINLLE AYKMSNKTIA FALVVASSAP VIAADNDNIM VVTASGYEQK IREAAASISV ISQNELRQRN YNDLAQALSD VEGVDVNSST GKTGGLDISI RGMPSAYTLI LVDGIRQNGT SDVTPNGFGA MNTSFMPPLS AIERIEVIRG PMSTLYGSDA IGGVVNIITK KITKAWASSA TLEHTFQENT AFGDSSKFSF YSSGPAVEDQ LGLSLRGTIF RRDASRVESS NTGVELSRRG PNPVKADNYN LGGKLFWQLN TQSTLWLDGD IANQKYDNSA NQLGTIGARG GYEDTLRYQR RKITLGNDNR LDFGTWNSSL SYNQTENIGR LITNASVPQG SGLAGEKRLL KNTNIILDSK LVAPLGDNHM VTLGGQYWNA IMKDGIVLAN NGDEFAQDAW SLFSEDEWRL LDSLALTYGA RYEYQTTFGG HISPRAYLVW DAQDNWTVKG GVSTGYKTPT LAQLHNGISG VTGQGTITTI GNPKLEPESS VNTEVGVYYE NETGFGANVT LFHNRFRNKI NSVSIDNTTS TYTNVGKAIT QGIEVASTIP LWSDDWMLGI NYTFTDSEQK DGNNKGARLT NTPKNMVNAR LNWNINEQLS TWLKAEYRSK TARFTQNYAN LSAANKVVYN NLGSEFKPFS VLNLGVAYKV TKDVTLNGAV NNLLDKDFTR THIFAVGNGT TTAGDYFTSS QSTAGYVVPG RNYWVSVNVN F  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGATAATTGATATCATTTGCTTTT  

PCR Backward Primer

TTATCCACTTCCAATGCTAGAAATTAACATTCACGGATA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011