Clone

Target

IDP90983  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

751  

Reference Sequence Used

MLKNSCLLVK GDLYMLKKIL PVLITLAIVH NTPTAWAAEA PKTDSFYLPK SLDLSPLRLH NIESNPYGKD FNYAQQFKTL DLEAVKKDIK TVLTTSQDWW PADYGNYGPF FIRMAWHGAG TYRIYDGRGG ADGGQQRFEP LNSWPDNANL DKARRLLWPI KKKYGAKISW GDLMVLTGNV ALESMGFKTL GFAGGREDDW QSDLVYWGAG NKMLSDNRDK NGKLPKPLAA TQMGLIYVNP EGPNGKPDPV AAAKDIREAF ARMAMNDEET VALIAGGHTF GKAHGAASPE KCLGAAPGEA GLEQQGLGWA NKCGSGNGKD TITSGLEGAW TTDPTHFTMQ YLSNLYKHEW VLTKSPAGAW QWKPKNAANV VPDATDPTKF HPLMMFTTDI ALKVDPEYKK ITTRFLENPE EFKMAFARAW FKLTHRDMGP AARYLGDEVP KETFIWQDPL PAANYKMIDS ADISELKDKI LKTGLSDTKL IKTAWASAST FRGTDFRGGD NGARIRLAPQ KDWPVNDPAE LHSVLAALME VQNNFNKDRS DGKKVSLSDL IVLGGNAAIE DAAKKAGYSI SIPFTPGRTD ASQEETDVSS FAVLEPTADG FRNYYDAKRN TLSPIASLID RANKLELTVP EMTVLIGGLR VLDVNSGGSK AGVLTNTPGQ LNNNFFVNLL DMSTKWTKSP KAEGYFDGYD RKTGKLKWTA SSVDLVFGSN PELRAVAEVY ASDDAKEKFV HDFTKVWEKV MNLDRFDIKN N  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTAAAAAATAGTTGCCTTTTAG  

PCR Backward Primer

TTATCCACTTCCAATGTTAGTTATTTTTTATATCAAAGCGA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011