Clone

Target

IDP90990  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

716  

Reference Sequence Used

MATTKLNVID SSDSELDYHS LNAMLNLYDA DGRIQFDKDR LAARQYFLQH VNQNTVFFHN LQEKIDYLIE EGYYEKEVLD QYDFSFIKSL FQQAYSKKFR FPTFLGAFKY YTSYTLKTFD GKRYLERYED RVCMVALTLA TGNQTLAREL VEEIISGRFQ PATPTFLNCG KKQRGELVSC FLLRIEDNME SIGRSINSAL QLSKRGGGVA FLLSNIREVG APIKRIENQS SGVIPIMKML EDAFSYANQL GARQGAGAVY LHAHHPDILR FLDTKRENAD EKVRIKTLSL GVVIPDITFE LAKNNEEMYL FSPYDIEQVY GVPMSEISIS EKYREMVDNK RIRKSKIKAR EFFQILAEIQ FESGYPYMMF EDTVNRANPI KGRINMSNLC SEILQVNAPT EYHEDLSYRR IGKDISCNLG SLNIAQVMDS PDFGKSIEIA IRGLTAVSEM SNIRSVPSIE KGNQESHAIG LGQMNLHGYL ARERLFYGSE EALDFTNIYF YTVAYHAIRA SNQLAIERAS HFKGFDESDY ASGAFFSKYI EQSWQPKTAR VQALFADAGI AIPNQQDWIA LRQSVMEHGI YNQNLQAVPP TGSISYINHS TSSIHPIVSP IEIRKEGKIG RVYYPAPYMT NDNLEYYQDA YQIGPEKIID TYAAATQHVD QGLSLTLFFR DTATTRDINK AQIYAWKKGI KTIYYIRLRQ MALEGTEVEG CVSCSL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCAACGACAAAACTGAAC  

PCR Backward Primer

TTATCCACTTCCAATGTTACAGCGAACAGGAAACGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011