Clone

Target

IDP00228  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

441  

Reference Sequence Used

MAAVVGVVTG LLGVAFDRGV DWVQQQRLLA LANVADYALL VWPLAFIMSA LLAMMGYFLV SRFAPEAGGS GIPEIEGAME EMRPVRWWRV IPVKFIGGLG TLGAGMVLGR EGPMVQMGGN SGRMIVDIFR LRSPEARHSL LATGAAAGLS AAFNAPLAGI LFVIEEMRSQ FRYSLVSIKA VFIGVITSTI VYRYFNGERA IIEVGKLSDA PLNTLWLYLL LGIIFGAVGV IFNALIFRTQ DMFVRFHGGD WRKLVLIGGL LGGMCGLLAL LHGNAVGGGF ALIPIAAAGN FSIGMLLFIF IARVITTLLC FGSGAPGGIF APMLALGTIL GTAFGLSCAH FFPQYGIEAG TFAIAGMGAL FAASVRAPLT GIVLVLEMTD NYQLILPMIV TCLGATLIAQ FMGGKPLYSA ILARTLAKQE QARATVIAQE PAVENTPQIG K  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCCGCTGTTGTCGGTG  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTGCCTATTTGTGGGGTA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008