Clone

Target

IDP91006  

Clone Suffix

1 The clone is not available in BEIR.

Sequence Comments

87: D -> G  

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

441  

Reference Sequence Used

MAGLPTNSNV LERQRLEQQR QEQQRIDALK HFGQLFKQRQ IEPSADARAH WQQVLQLGFP GSKHEDWKYT PLERLFAHEF SFPHAPDVTT AQCDALSLVP NAHRLVFING QFSPTLSDRE CGPYQLTHAA ENVPFPTAIQ SEVFLHLTES LAQERLHIRL PVGQHSDKSL YLLHISSGND SDMVNTSHSR HHLVVEANAQ AEVIEHFVSL NEQPHFTGAR LTIAVGDNAE LNHYKLAFET PKSYHFAHND LVLGRDARAR SHSFLLGAGL TRHNTSSQLN GEGSSLSMNS LLLPVGREIC DTRTYLEHNK GHCESRQLHK VMVRERGKAI FNGMIKVAKH ALKTDGQMTN NNLLLSHLAE VDTKPQLEIY ADDVKCSHGA TVGRIDAEQL FYLQSRGINQ ADAQQMIIFA FAAEVTEAIH NEAIRKQVLA RIAERLAWES V  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MAGLPTNSNV LERQRLEQQR QEQQRIDALK HFGQLFKQRQ IEPSADARAH WQQVLQLGFP GSKHEDWKYT PLERLFAHEF SFPHAPGVTT AQCDALSLVP NAHRLVFING QFSPTLSDRE CGPYQLTHAA ENVPFPTAIQ SEVFLHLTES LAQERLHIRL PVGQHSDKSL YLLHISSGND SDMVNTSHSR HHLVVEANAQ AEVIEHFVSL NEQPHFTGAR LTIAVGDNAE LNHYKLAFET PKSYHFAHND LVLGRDARAR SHSFLLGAGL TRHNTSSQLN GEGSSLSMNS LLLPVGREIC DTRTYLEHNK GHCESRQLHK VMVRERGKAI FNGMIKVAKH ALKTDGQMTN NNLLLSHLAE VDTKPQLEIY ADDVKCSHGA TVGRIDAEQL FYLQSRGINQ ADAQQMIIFA FAAEVTEAIH NEAIRKQVLA RIAERLAWES V  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCTGGCTTACCGACCA  

PCR Backward Primer

TTATCCACTTCCAATGTCATACAGACTCCCACGCC  

Shipped To Lab

ANL  

Shipped Date

Sep 29, 2011  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011