Clone

Target

IDP00182  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

348  

Reference Sequence Used

MIVEGYYQYP GRAAREAARL LLNEIGDNVT LLRDGEQHYF SLSQLNVSSS LGSIPLNLTF PDGGRFVPSD DALFRKWLYQ QRSPGVVHRL ERHKSGVLLT LLITPLLILA YIYWLLPWAS SEIALRIPTT AEQKLGEHSL EWLQQSGFKP SALPAERQEA IQQLFLQVMP PQMRQERTPL KLVLMTAPTT ANAFMLPDGT MVISDDLIKL TTSDDALAAV MLHEMGHHAH RHSMQMVVRS SLVALTFMWM TGDVNGIGDT LLQSAAFIHE MQFSRTIESE ADSWAIAEMQ REKRSLLAME DIYHALQQTT TPEEAETRAG IPEWLSSHPD MPARLDVIRA AREKMAQE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGATCGTCGAAGGATATTATC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTCCTGCGCCATCTTCTC  

Shipped To Lab

NU  

Shipped Date

Jul 16, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008