Clone

Target

IDP91013  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

failed  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

673  

Reference Sequence Used

MAKLLIFIKS LIVGMMIVST MGCAEKPTFV PPAQRALPQS ERPWQKNTFV VIAYHDVEDD SADQRYLSVR SSALNEQFVW LRDNGYHVVS VDQILAARNG GPTLPDKAVL LTFDDGYSSF YRRVYPLLKA YKWSAVLAPV GTWIDTATDK KVDFGGLSTD RDRFATWKQI TEMSKSGLVE IGAHTYASHY GVIANPQGNT EPAAANLQYD PKTKQYETVE AFKQRMEKDV ALITQRIVQA TGKQPRVWVW PYGAPNGTVL NILRQHGYQL AMTLDPGVAN INDLMNIPRI LISNNPSLKD FALTVTSVQE KNIMRVAHVD LDYLYDPDPA QEKENLDKLV QRISDLRVTH VFLQAFSDPK GDGNIRQVYF PNRWIPMRQD LFNRVVWQLA SRPDVEVYAW MPVLAFDMDP SLPRITRIDP KTGKTSIDPD QYRRLSPFNP EVRQRIIDIY RDMAYSAPID GIIYHDDAVM SDFEDASPDA IRAYEKAGFP GSITTIRQDP EMMQRWTRYK SKYLIDFTNE LTREVRDIRG PQVKSARNIF AMPILEPESE AWFAQNLDDF LANYDWVAPM AMPLMEKVPL SESNEWLAEL VNKVAQRPGA LEKTVFELQS KDWTQPEGNN AISGPILAGW MRQLQLSGAQ SFGYYPDNFI TGEPPLKDVR PVLSSAWYPL YDR  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCGAAGCTATTAATTTTTATC  

PCR Backward Primer

TTATCCACTTCCAATGCTATCGATCATATAAAGGGTAC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011