Clone

Target

IDP00177  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

509  

Reference Sequence Used

MAVLQIFDNF IQQTDNLAHD KMNNRGTTLR VLHRGTIVLL KPAILIKTDM LIKPDILIKP ATLIKQAARI KQMTLLLLLF LFSLFGVAKA ATQVVNVKSE LAAPVMLANS EDKNYLKISL TGFNLDSTRR SPINLALVID RSTSMSGERI EKAREAAILA VNMLNITDTL SVVAYDNHAE VIIPATKVTD KPALIASIQQ HIHPRGMTAL FAGVSMGIGQ VDKHLNREQV NRIILISDGQ ANTGPTSISE LSDLARMAAK KGIAITTIGL GQDYNEDLMT AIAGYSDGNH TFVANSADLE KAFTKEFQDV MSVVAQDIVV QIKTGDKVKP VRLLGRDGDI LGNTVNVKLN QLYSNQEKYI LLEVIPEKGT DKQQKDLADV SISYLNLSSK KQDQINERVT VSYSQSVEKV NDAVQEEVLA ESEIQKTALA NDEAIKLIDA GRKDEAKKVL ESNASKLDSM SFSSPVAEKK VRESTNKQRK LADDIDSKDA ATYRKELKEQ NYNVKQQQK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCCGTTTTACAAATTTTTGA  

PCR Backward Primer

TTATCCACTTCCAATGTTACTTCTGCTGCTGTTTGAC  

Shipped To Lab

NU  

Shipped Date

Jul 16, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008