Clone

Target
IDP91328  
Clone Suffix
1
Experiment Start Date
Jul 22, 2011  
Experiment End Date
Aug 01, 2011  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
105  
Reference Sequence Used
MKVAVVVLSL LALGVIVKNS KSNLVDLTKP RGVRANNPLN IEQNRSNNWL GKVTPSVDKR FETFSAPKYG FRAGARTLRT YQNKHGLNSI RELIHRFAPS NENKK  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAAAGTTGCGGTGGTTGTT  
PCR Backward Primer
TTATCCACTTCCAATGTCACTTTTTGTTTTCATTGCTTG  

Related Expressions

           
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2011-08-02 2011-08-02 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-11-04 2011-11-04 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2012-09-13 2012-09-15 Katarzyna Handing University of Virginia uva_expression_native_1 success E.coli LB IPTG production
2012-11-27 2012-11-29 Katarzyna Handing University of Virginia uva_expression_native_1 success E.coli LB IPTG production
2012-11-27 2012-11-29 Katarzyna Handing University of Virginia uva_expression_native_1 success E.coli LB IPTG production
2011-08-24 2011-08-24 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay