Clone

Target

IDP00226  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

815  

Reference Sequence Used

MTSPFSYTSP VVSVDALKHS IAYKLMFIIG KDPTIATQHD WLNATLFAVR DRMVERWLRS NRAQLSQDVR QVYYLSMEFL LGRTLSNALL SMGIYDEIEQ ALDEMGLSLS ELLKEENDPG LGNGGLGRLA ACFLDSLATL ALPGRGYGIR YEYGMFSQKI VNGQQMESPD NWLEYGNAWE FPRHNTRYKV RFGGRIQQEG SKIRWLETEE ILACAYDQII PGFDTDATNT LRLWSAQASN EINLGKFNQG DYFAAVEDKN HSENVSRVLY PDDSTYSGRE LRLRQEYFLV SATVQDILNR HWAMHHTFNN LADKIAIHLN DTHPVLSIPE MMRLLIDEHK FTWMDAWDVV QQVFSYTNHT LMSEALETWP VDMIGKILPR HLQIIFDIND HFLKLVEEQY PDDKELLSRV SVIDENNGRR IRMAWLAVIA SHKVNGVSAL HSELMVQSLF ADFARIFPNR FCNKTNGVTP RRWLGLANRP LAAVLDDSIG QTWRTDLSQL SELEKNLDYP SFLLALQKAK LENKKRLAVY IAEKLNIVVN PAALFDVQIK RIHEYKRQLL NVLHVITRYN RIIDAPDNNW VPRVVIFAGK AASAYYNAKQ IIHLINDVAK VINNDPRINN LLKVVFIPNY SVSLAQLIIP AADLSEQISL AGTEASGTSN MKFALNGALT IGTLDGANVE IREHVGEENI FIFGNTTEQV EALRKSGYNP RKYYDEDPEL HQVLTQIATG TFSPEEPHRY TNLFDSLVNL GDHYQLLADY RSYVDTQEQV DALYRNRDEW SRKTLLNIAN MGYFSSDRTI KEYADEIWHI KPIRL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGACATCACCGTTTAGCTATA  

PCR Backward Primer

TTATCCACTTCCAATGCTACAGCCGGATAGGCTTAA  

Shipped To Lab

NU  

Shipped Date

Jul 16, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008