Clone

Target

IDP00222  

Clone Suffix

3

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

589  

Reference Sequence Used

MKALRRKWQS LPRGLVVLIT ALVIYIPISF IIIQSFLSAP FFSPSKVLSL DAFRFIFSDP DFYKALKSGF ILATGLVVIA IPLGGILAFL MIRTDLPGRR IIEPLILVPI FVSPMVLGFG YVVAAGPVGF FSLWAESLLG FVPWNIYAMS SIVVIAGLTH VPHAYLYISS ALRSVGSDVE EAARTAGASP LQVMSAVSLP MVRPSILYAT VLLFFLGLEV FGLMLVLGDP EGNLVLATYL YQLTNKLGTP SYHLMAAVAV VLICITIPLV MLQHMLMRTA HRFVTVKGKA SQARALPLGK WRWVAGAVVA FWLTVTIGVP LVGVVLRAFV SNWGVGVSLW DELSLSTFYT IWQQPNLLRA IVNSMAIGVF GGALAVICYL FIGIAMHRKP DGVTRFLDYS VLVPRAVPGL LAGLAFLWVF LFLPMWLDNS LKEGWLAALP MTEWLQDHLV VWLRSLRSTI FSVWLAYTVV WMAYGLRLIS STLLQVGPEL EEAARSTGAR RGQVTRHVTI PLSRYGLIGS WLLMFLIFER EYSTGVYLLS PGTETIGSML VSLWAAGAID IVAALSFINI LLVVLGLGIA LRFGVKLHD  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAAGCATTGCGCAGAAAAT  

PCR Backward Primer

TTATCCACTTCCAATGTCAATCATGTAATTTGACTCCAA  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008