Clone

Target

IDP91424  

Clone Suffix

2

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

591  

Reference Sequence Used

MDKQFAWIAH LAEHYGAIIL MAVFGYVVAR LLHLRYQREL DTLILDLMSP VLLVDLTGQH VLLANSAAMQ QLGVRQLNRT YLFPATFAIE QLSNSLTSLS GRQFSQLAFA WQASEHEHLD VALSGRKVYY RGQHCWLLNI QLNPSNRSRH MDDLDAFLVA QSALDSLSEL ICLKDNDGKI LATNRAFELF WEGRRDEAIM PESRLMKGRQ SERRWTTDPQ GRSCLLEVNQ SLLMTKAGEP IGTLSISHDV TEWHAMQQNL RDEMERRKDT EVALAQRDTI LQNILEASPD SIGIFNENLV YQACNEPFVR ALGIADVNDL IGKRLQDVVP GEMYQRLSVT DSQVLHEGKS LRYIDKVLDS NGEYAWYDVV KSPFRDPASG TNGVLIMARD ISERYLAEQK LEAANLELEK LSFMDSLTQV SNRRRFDEQL NTLWFHHARE KLPLTIMLCD IDFFKEFNDR YGHQAGDEAL IQVATVFSQV VSRSSDCVAR YGGEEFALLL PNTTAEGALK VAEKIHAYIA ESNIAHQGSK IAGRVTISIG LVSYIPSPED VPDMGVALAD SALYQAKSDG RNRTCVHPIS LSSGESPSTL A  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGGACAAGCAGTTTGCATGG  

PCR Backward Primer

TTATCCACTTCCAATGCTATGCCAAAGTGGACGGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011