Clone

Target

IDP91458  

Clone Suffix

2 The clone is not available in BEIR.

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

429  

Reference Sequence Used

MADKKATLHV EGKAPIELPI MEGSLGTPVI DVRKLGANGY FTFDPGFLAT ASCESQITYI DGGKGILLHR GYPIDQLANN ADYLEVCYIL LYGEAPTREQ YEKFKTTVTR HTMVHEQIAS FFHGFRRDAH PMAVMCGVVG ALAAFYHDSL DINNDLHREI TAYRLLSKMP TLAAMCYKYS TGQPFIYPRN DLSYAENFLH MMFATPCEEY EVNPVVARAM DKIFTLHADH EQNASTSTVR LAGSSGANPF ACIAAGIASL WGPAHGGANE ACLKMLEEIG SVDNIPEYVD RAKDKDDPFR LMGFGHRVYK NYDPRATVMR ETCHEVLKEL NIQDPLLDVA MELERIALSD EYFVSKKLYP NVDFYSGIIL KAIGIPVSMF TVIFAISRTI GWIAHWNEMH SDPLNRIGRP RQLYTGEVQR DFQPMHERE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MADKKATLHV EGKAPIELPI MEGSLGTPVI DVRKLGANGY FTFDPGFLAT ASCESQITYI DGGKGILLHR GYPIDQLANN ADYLEVCYIL LYGEAPTREQ YEKFKTTVTR HTMVHEQIAS FFHGFRRDAH PMAVMCGVVG ALAAFYHDSL DINNDLHREI TAYRLLSKMP TLAAMCYKYS TGQPFIYPRN DLSYAENFLH MMFATPCEEY EVNPVVARAM DKIFTLHADH EQNASTSTVR LAGSSGANPF ACIAAGIASL WGPAHGGANE ACLKMLEEIG SVDNIPEYVD RAKDKDDPFR LMGFGHRVYK NYDPRATVMR ETCHEVLKEL NIQDPLLDVA MELERIALSD EYFVSKKLYP NVDFYSGIIL KAIGIPVSMF TVIFAISRTI GWIAHWNEMH SDPLNRIGRP RQLYTGEVQR DFQPMHERE  

PCR Forward Primer

TACTTCCAATCCAATGCGATGGCGGATAAGAAAGCGAC  

PCR Backward Primer

TTATCCACTTCCAATGTTACTCGCGTTCGTGCATAG  

Shipped To Lab

ANL  

Shipped Date

Sep 29, 2011  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011