Clone

Target

IDP91468  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

484  

Reference Sequence Used

MLKIKLPQQT SLAPSSETTQ RLPVKISIKS ICNKSICKTL HSLADKCHRF SKEIKQRSAN HTPSSSHENL TTINLKSQNS AATFESTVEL SIKHGSSIPP APPLPGAIPP APPLNLSKGA PKSSSNSLDS VNEDHSKLME QIRQGVKLKS ATKSLSADKS SADAHSKLME ELLTGGRKLK KVATSDIPAP PPLPSASTSK SPDSRNALLS EIAGFSKDRL RKTGSLETLN SSQSKDKESF EPTTHERLLS EDLFKQSPKL SEQELDELAN NLADYLFQAA DIDWHQVISE KTRGLTTEEM AKSEHRYVQA FCREILKYPD CYKSADVASP ESPKSGGGSV IDVALKRLQT GRERLFTTTD EKGNRELKKG DAILESAINA ARMAISTEEK NTILSNNVKS ATFEVFCELP CMDGFAEQNG KTAFYALRAG FYSAFKNTDT AKQDITKFMK DNLQAGFSGY SYQGLTNRVA QLEAQLAALS AKLS  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTTAAAATTAAACTGCCTCA  

PCR Backward Primer

TTATCCACTTCCAATGCTACGATAATTTTGCAGATAGTG  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011