Clone

Target
IDP91511  
Clone Suffix
1
Experiment Start Date
Jul 22, 2011  
Experiment End Date
Aug 01, 2011  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
5206  
Reference Sequence Used
MGKPFWRSVE YFFTGNYSAD DGNNSIVAIG FGGEIHAYGG DDHVTVGSIG ATVYTGSGND TVVGGSAYLR VEDTTGHLSV KGAAGYADIN KSGDGNVSFA GAAGGVSIDH LGNHGDVSYG GAAAYNGITR KGLSGNVTFK GAGGYNALWH ETNQGNLSFA GAGAGNKLDR TWFNRYQGSR GDVTFDGAGA ANSISSRVET GNITFRGAGA DNHLVRKGKV GDITLQGAGA SNRIERTRQA EDVYAQTRGN IRFEGVGGYN SLYSDVAHGD IHFSGGGAYN TITRKGSGSS FDAQGMEYAK AEDIVLTAAQ MHGLSIDNGN KFHAVTAVKS EREPNTYLFA IADGTYTKIN KVRLYNDPET GKLKYYSEAW FKRGNHLAEL ARSDVSSAGG FEVNPINGGY TLSNIAVEHQ QSLTVHAVEK DLTEYEWVTY ANGALIDAKD VALSEAKMGG HAISTDGTTV DVQAVKSNRK PNTYVYAKVL GPYTKIVVVE LANDPKTGAL KYQARSWYKE GNHTANLANE DISSANGYHS MGKGGYSLSD LHYSVNAVRS TSETVADIDE YTDQTLFKPA TDSGESSGDV RFNGAGGGNV IKSNVTRGNV YFNGGGIANV ILHSSQFGHT EFNGGGAANV IVKSGEEGDL TFRGAGLANV LVHQSKQGKM DVYAGGAVNV LVRIGDGQYL AHLLAYGNIS VHKGNGNSRV VMLGGYNTHT QIGSGNGLWL AAGGFNVMTQ VGKGDVASVL AGGANVLTKV GDGDLTAGML GGANVITHIS GDNETSNTTA VALGGANILT KKGKGNTLAV MGGGANVLTH VGDGTTTGVM VGGANILTKV GNGDTTGIML GVGNVLTHVG DGQTLGVMGA AGNIFTKVGD GTSIAVMIGA GNIFTHVGEG NAWALMGGLG NVFTKVGNGD ALALMVAEAN VFTHIGDGMS VALMLAKGNV ATKVGNGTTL AAMVGNANIF THVGSGSTFA AMIGQANIMT KVGNDLTAAL MVGKANIYTH VGDGTSLGIF AGEVNVMTKV GNGTTLAAMF GKANIMTHVG DGLTGVLALG EANIVTKVGD DFMGVVAAAK ANVVTHVGDA TTAAVLAGKG NILTKVGEGT TVGLLISDIG NVMTHVGDGT TIGIAKGKAN IITKVGDGLG VNVAWGQANV FTQVGDGDRY NFAKGEANII TKVGDGQEVS VVQGKANIIT HVGNGDDYTG AWGKANVITK VGNGRNVVLA KGEANIVTQV GDGDSFNALW SKGNIVTKVG DGMQVTAAKG KANITTTVGD GLSVTAAYGD ANINTKVGDG VSVNVAWGKY NINTKVGDGL NVAVMKGKAN ANIHVGDGLN INASYAQNNV AIKVGNGDFY SLAVASSNTS SNKLSALFDN IKQTLLGVGG SQAINYLVQG DEASSSGTQK GRGAIATPEI TKLDGFQMEA IEEVGSDLGD SLTGSVTKVD TPDLNKMQNA LDVDGSADQT QAPNLIVNGD FEQGDRGWKS THGVEASYSG NVYGVNGEGH GARVTELDTY TNTSLYQDLT DLTEGEVIAV SFDFAKRAGL SNNEGIEVLW NGEVVFSSSG DASAWQQKTL KLTAHAGSNR IEFKGTGHND GLGYILDNVV AKSESSQQAN AVSEHATQNQ ASQNALSDKE RAEADRQRLE QEKQKQLDAV AGSQSQLEST DQQALGNNGQ AQRDAVKEES EAVTAELTKL AQGLDVLDGQ ATHSGESGDQ WRNDFAGGLL DGVQSQLDDA KQLANDKIAA AKQAQSDNNS KVKESVAKSE AGVAKGEQNR AGAEQDIAEA KADAETRKAD AVAKSNDAKQ AESDAHSAAN DAQSRGDRDT MNAENKANQA QNDAKGTKQN EGDRPDREGV AGSGLSGNAH SVEGAGETGS HVTTDSQTNA DGRFSEGLSE QEQEALEGAT NAVNRLQINA GIRGKNSGST ITSMFTETNS DSIVVPTTAS QDLVRKEIRI SGVNLEGLGE TSHDSAESLV AARAEKVANL YRWLDTDNDV ATDKYVPVPG FERVDADVSD EVKQRMIQSM SGYIEHTDNQ VPKDQAEALA TLFVEATLDY DWDKRVEFLT KLESYGYSFE APHAEKSIVS FWSGKNFKQY RDVLDNAQTD GKKVVYDIDV KGNAFAIDLN KHLMRWGGLF LDPDNAEQNQ LKSSIDAATF SNTGFWSSVY ATGAQNDVYV IAEGGVRLGN YFWNVELPAL RQLQREGLVG EIRLLDKPVS EYKDLPADQI GRRLTDAGVA VKVRFDALSH ERQAELLADN PDGYKADTLV ELDVKLSAID SMLRESLPFY SLRTERNLLV QEGEEGFEVR SWPGIDGKSK TILLDNPEDA AQQKSIERFI LANFDNFEQM PDELFLVDNK VLSHHDGRTR IIVQKEDGAW TYNTNVELMS VTELLDAAHV NGKVRGDSYQ QVIDALTEYH ASTVEHADYE LESVEKLLNL RKQIEGYVLG HPDSGRVEAM NSLLNQVNSR LEEVSVLAVS EQSIKAHDSF SRLYDQLDNA NLKESKHLYL DGNGDFVTKG KGNLATIDQL GGSDAVLEKV KAAVTHEYGQ VVADTIFAGL SANDLAKDGK GIDIAGLNKV HQAIEQHMSP VSATMYIWKP SDHSALGHAA LQIGQGRTQL EGQAAADFNK QNYVSWWPLG SKSSNIRNIF NVATEDQPDL KLRWSDFSQP AHQNDTLEHD MASEENDGFG LKDGETKLKR FIEKLNAAKG IDASYKDASE GYASVLLGNP DMLASTGIPA HVFQPFVDQW NDTSYDMMDV ANRFAEELQK QAQASGDPAL VEKRIDNVVR LFAERALEEI EAFKASQADE GRVFRINLEG LDVAAMQAEW NRLSNDPDAR YQLLTKNCSS TVAKVLKAGG ADKLIGHTWR PKFGVWTPTE LFNFGQALQE AQLEIAAKKQ SHQVTDVLDA LSGNEKHKEN VAIENDGTPP RDKESLSPLT RFLNNELYGE KDARRKIGEI TQTLLDHAVE NGESQKVTLK GEAGRLTGYY HQGAVSSEGE TSATSGKVVL FLHGSGSSAE EQASEIRNHY QKQGIDMLAV NLRGYGESDG GPSEKGLYQD ARTMFNYLVN DKGIDPSNII IHGYSMGGPI AADLARYAAQ NGQAVSGLLL DRPMPSMTKA ITAHEVANPA GIVGAIAKAV NGQFSVEKNL KGLPKETPIL LLTDNEGLGE EGEKLRAKLA IAGYNVTGEQ TFYGHEASNR LMGQYVDQIV SGLFNAEQAA VEAGEVLKGL EKDFKRYGDA LKPDTSVPGK SKDIRTTKDF LNGYKNDHAK EIVDGFRSDM SIKQLVDLFV KGNWSAEQKG ALAWEIESRA LKVTFQNKSE KYNRLFREIA SAGVVDAKAT EQLAPQLMLL NLSNDGFGGR CDPLSKLVLV AKQLENDGQV GVARQLLEKM YSAAAVLSNP TLYSDSEKAN ASKLLSSLAA IHAKNPMHDT SMKVWQEKLE GKQALTVNGV VEKITDASAN GKPVLLELDA PGHAMAAWAK GSGDDRVYGF YDPNAGIVEF SSAEKFGDYL TRFFGKSDLN MAQSYKLGKN DAGEAIFNRV VVMDGNTLAS YKPTFGDKTT MQGILDLPVF DATPIKKPTG GVASDLEALG DKTKVVVDLA QIFTVQELKE RAKVFAKPIG ASYQGILDQL DLVHQAKGRD QIAASFELNK KINDYIAEHP TSGRNQALTQ LKEQVTSALF IGKMQVAQAG IDAIAQTRPE LAARIFMVAI EEANGKHVGL TDMMVRWANE DPYLAPKHGY KGETPSDLGF DAKYHVDLGE HYADFKQWLE TSQSNGLLSK ATLDESTKTV HLGYSYQELQ DLTGAESVQM AFYFLKEAAK KADPISGDSA EMILLKKFAD QSYLSQLDSD RMDQIEGIYR SSHETDIDAW DRRYSGTGYD ELTNKLASAT GVDEQLAVLL DDRKGLLIGE VHGSDVNGLR FVNEQMDALK KQGVTVIGLE HLRSDLAQPL IDRYLATGVM SSELSAMLKT KHLDVTLFEN ARANGMRIVA LDANSSARPN VQGTEHGLMY RAGAANNIAV EVLQNLPDGE KFVAIYGKAH LQSHKGIEGF VPGITHRLDL PALKVSDSNQ FTVEQDDVSL RVVYDDVANK PKITFKGSLS GANTALHNQN VNDWERVVVT PTADGGESRF DGQIIVQMEN DDVVAKAAAN LAGKHPESSV VVQIDSDGNY RVVYGDPSKL DGKLRWQLVG HGRDDSESNN TRLSGYSADE LAVKLAKFQQ SFNQAENINN KPDHISIVGC SLVSDDKQKG FGHQFINAMD ANGLRVDVSV RSSELAVDEA GRKHTKDANG DWVQKAENNK VSLSWDEQGE VVAKDERIRN GIAEGDIDLS RIGVSDVDEP ARGAIGGNND VFDAPEKRKA ETETSSSSAN NKLSYSGNIQ VNVGDGEFTA VNWGTSNVGI KVGTGGFKSL AFGDNNVMVH IGNGESKHSF DIGGYQALEG AQMFIGNRNV SFNLGRSNDL IVMMDKSIPT PPLVNPFDGA ARISGVLQSI ATSGEGQDWL AAQEQQWTLS GAKKFVKDMS GLDQSSSVDY TSLVELDSQN ERSSRGLKHD AEAALNKQYN QWLSGNSDSD TSKLSRADKL RQANEKLAFN FAVGGQGADI QVTTGNWNFM FGDNIQSILD TNLGSLFGLM TQQFSATGQA KTTFTYTPED LPRQLKNKLL GQLAGVGAET TLADIFGVDY TASGHIVSRN GEAVDGVAIL KEMLEVIGEF SGDQLQAFVD PAKLLDSLKS GINMGADGIK SFAETHGLKE KAPEEEEDNS SVSVNGANVN SAQGATVADG STETAETPDR AFGFNSLNLP NLFATIFSQD KQKEMKSLVE NLKENLTADL LNMKEKTFDF LRNSGHLQGD GDINISLGNY NFNWGGDGKD LGAYLGDNNN FWGGRGDDVF YATGTSNIFT GGEGNDMGVL MGRENMMFGG DGNDTAVVAG RINHVFLGAG DDQSFVFGEG GEIDTGSGRD YVVTSGNFNR VDTGDDQDYS VTIGNNNQVE LGAGNDFANV FGNYNRINAS AGNDVVKLMG YHAVLNGGEG EDHLIAAAIS KFSQFNGGEG HDLMVLGGYQ NTFKGGTDVD SFVVSGDVID NLVEDIRSED NIVFNGIDWQ KLWFERSGYD LKLSILRDPA SDSDQAKFEH IGSVTFSDYF NGNRAQVIIA MGEKDATGER EYTTLSESAI DALVQAMSGF DPQAGDNGFI DNLDSKSRVA ITTAWADVVH KKGITV  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGGGAAAACCATTTTGGAGAA  
PCR Backward Primer
TTATCCACTTCCAATGTTACACCGTTATACCCTTTTTAT