Clone

Target

IDP91551  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 21, 2011  

Experiment End Date

Jul 21, 2011  

Person

Sofiya Shatsman  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

504  

Sequence End

850  

Reference Sequence Used

IRRKIPVKTF DECSEYVEKI IEEYKGDSLT HLVFLKDKYI YLNEDKDLFI QYEVYGDKLF VLGNPVGNNE NLFREIEKFC EYADNYGYTP VFYQVNDEMI SYLHSNGYDF MKIGEEAKVD VKEFKVVGNK MKSLKTSRSK VTKEGYTFHM VEPPFSKEFL DSLREISDEW LDGRKEKGFS VGFFDEDYLN KAPIAILKDR EGEIKAFANI MYMYDDESFS VDLMRFSKNT PRGVMDFMFI NLIEYGKEKG YEIFNMGMAP LANVGLSKYA FWNEKLALQF YENGQALYSF KGLRRFKEKF SHNWEYKYIA YRRNTSILIT VIQAAIVCSR NRNVDESIVV RNLKSLI  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATAAGAAGAAAAATTCCTGTTAAG  

PCR Backward Primer

TTATCCACTTCCAATGTCAAATTAAGCTTTTTAAGTTTCTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011