Clone

Target

IDP91566  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jul 22, 2011  

Experiment End Date

Aug 01, 2011  

Person

Sarah Grimshaw  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

453  

Reference Sequence Used

MLPTSQLRPT GTFCSYSAET SADIKSEITP IQIEEARASG RLYIKDCDIE YLPQLPNEIT SVTIENCNNL TTLTGLPVNT QNLSVINCEK LQITDMPSTV KNLHIELTDS PFIHFISEGI ECLTVCHCYI SGVPESVRYL EIKGSATDSI KNVPNGLSSL SINSYNPENQ ARIDNLISPS LKTLSLTGCS NIILPEKLPE SVTSVTIHAE QKTTWNIGVE GMPDGLDLDL QNVLLSPDVV KAKNITFQGN ALDVALHFRE GDIVYGLSSP REKLVNSIKL VNDFSKKDII TQNTLTNAVW DPRTPRKYKQ DPLIKRALNE HERGIKFKQH LKNHNNYNVT MADLSVYNRD KLWAKTSKAG LEFQTLTRNK TVIFCADELV NSLKLIANKS EGYGQSITAS ELRWIYRNKD NNQIMKNIKF YLHGKEIPAE RILDTPEWKD YRPKYSGSTY KYS  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCTTCCTACATCGCAATTAC  

PCR Backward Primer

TTATCCACTTCCAATGTTAAGAATATTTATATGTGGAACC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 04, 2011