Clone

Target
IDP91586  
Clone Suffix
1
Experiment Start Date
Jul 22, 2011  
Experiment End Date
Aug 01, 2011  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
306  
Reference Sequence Used
MKVSQCGCGC AEIAGLKSLF QSLGCSLRSL NFNIPELLGL GFSGYNKTPL FKLCDSWMGR RLLYNRHFSN GLIQFLFSPH RENLVVYLRA FDVKIGPRWF HLCSEEEDLY FFSYVFKNAF CSTGLRAIRY EVKDLKEEIR TFLLRSEEGN MPRLSHFCCS PGGRKFLFVK PGLLNRILMT PENREQVQEA LLLPLSKGMC ALKNLDQGAI HWLMYSSWDD GKNFLKELWK NENAKHLLEE VLLRYKYNFS KKENETILDL LTIDVLEMKS ESSTTQAGNL GFFEQDHENK AEQETKENLQ FNRQPG  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAAGGTGTCCCAATGTGG  
PCR Backward Primer
TTATCCACTTCCAATGTTATCCAGGTTGTCTATTAAATTG  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2011-08-02 2011-08-02 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-08-24 2011-08-24 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay
2011-11-04 2011-11-04 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay