Clone

Target
IDP91593  
Clone Suffix
1
Experiment Start Date
Jul 21, 2011  
Experiment End Date
Jul 21, 2011  
Person
Sofiya Shatsman  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
271  
Sequence End
605  
Reference Sequence Used
YHKRTKPIGE KADPERLAAF LNEKGGNALS HLGFLGDKRF YFSSDGNALL LFGKIARRLV VLGDPSGQRE SFPLVLEEFL NEAHQKGFSV LFYQIEREDM ALYHDFGYNF FKLGEEAYVD LNTFTLTGKK KAGLRAINNR FEREEYTFHV DHPPFSDAFL EELKQISDEW LGSKKEKGFS LGFFDPSYLQ KAPIAYMKNA EGEIVAFANV MPMYQEGEIS VDLMRYRGDA PNGIMDALFI RMFLWAKEEG CTSFNMGMAP LANVGTAFTS FWSERFAAVI FNNVRYMYSF SGLRAFKEKY KPEWRGKYLA YRKNRSLSVT MFLVTRLIGK SKKDS  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGTATCATAAGAGAACGAAACCGA  
PCR Backward Primer
TTATCCACTTCCAATGTCAGGAGTCTTTTTTGCTTTTG  

Related Expressions

   
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2011-07-25 2011-08-02 Sofiya Shatsman J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-10-04 2011-10-04 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins failed E. coli 2xYT 1mM IPTG assay