Clone

Target

IDP00253  

Clone Suffix

301

Sequence Comments

NdeI-XhoI. Met at N-term added for expression in E.coli. Observed difference at position 438 ASN->THR. No tag.  

Experiment Start Date

Aug 26, 2008  

Experiment End Date

Sep 13, 2008  

Person

Chris Nelson  

Lab

Washington University  

Protocol ID

WU_basic_cloning_protocol_1  

Status

success  

Vector

pET21A(+)  

Sequence Start

286  

Sequence End

684  

Reference Sequence Used

MAHCIGITDR D FIEGVHGG TW VSATLEQ DKC VTVMAP DKPS LDISL ETVAI DRPA EVRKVC YNA VLTHVKI ND KCPSTGEA H LAEENEGDN ACKRTYSDRG WGNGCGLFG K GSIVACAK FT CAKSMSL FEV DQTKIQ YVIR AQLHV GAKQE NWTT DIKTLK FDA LSGSQEV EF IGYGKATL E CQVQTAVDF GNSYIAEMET ESWIVDRQW A QDLTLPWQ SG SGGVWRE MHH LVEFEP PHAA TIRVL ALGNQ EGSL KTALTG AMR VTKDTND NN LYKLHGGH V SCRVKLSAL TLKGTSYKIC TDKMFFVKN P TDTGHGTV VM QVKVSKG APC RIPVIV ADDL TAAIN KGILV TVNP IASTND DEV LIEVNPP FG DSYIIVGR G DSRLTYQWH KEGSSIGKL  

Reference Sequence Variance

 

Confirmed Protein Sequence

 

PCR Forward Primer

CGTCCATATGGCTCACTGCATTGGAATT  

PCR Backward Primer

CAGCTCGAGCTACAACTTTCCTATTGAGC  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Oct 02, 2008